pullorum strain h495  (ATCC)

Journal: PLoS Pathogens

Article Title: Cytolethal distending toxin induces the formation of transient messenger-rich ribonucleoprotein nuclear invaginations in surviving cells

doi: 10.1371/journal.ppat.1007921

Figure Lengend Snippet: Bacterial strains and cell lines.

Article Snippet: H . pullorum strain H495 ΔCDT , INSERM U1053, Bordeaux, France , CDT - , T6SS + , [ ] , [ ] .

Techniques: Plasmid Preparation

Journal: PLoS Pathogens

Article Title: Cytolethal distending toxin induces the formation of transient messenger-rich ribonucleoprotein nuclear invaginations in surviving cells

doi: 10.1371/journal.ppat.1007921

Figure Lengend Snippet: (A ) Images of liver Hep3B and colon SW480 cells following a 72h coculture. Cells were stained with fluorescent primary and secondary antibodies targeting UNR (green) and DAPI to counterstain the nucleus (blue). Fluorescent staining was observed using wide field fluorescence imaging . Yellow and white arrowheads indicate UNR-NR and cells undergoing mitosis, respectively. (B) Quantification of UNR-NR positive nuclei (%). At least 200 cells were counted for each experiment. Data represent the mean of triplicates in 1 representative experiment out of 3. ** p = 0.0003 and *** p<0.0001. (C) Coculture experiments with liver Hep3B were also performed using a 0.4-μm Transwell system. * p<0.0154 and *** p<0.0001 ΔCDT, CDT-knockout mutant strain; ΔCol., bacterial artificial chromosome; Col., pks genomic island encoding colibactin; DAPI, 4′, 6′-diamidino-2-phenylindol; E . c ., Escherichia coli; H . h ., Helicobacter hepaticus; NI, non-infected; WT, wild-type H . hepaticus strain 3B1.

Article Snippet: H . pullorum strain H495 ΔCDT , INSERM U1053, Bordeaux, France , CDT - , T6SS + , [ ] , [ ] .

Techniques: Staining, Fluorescence, Imaging, Knock-Out, Mutagenesis, Infection